野生大豆与栽培大豆杂交F1代ISSR多态性分析
ISSR Polymorphism Analysis of F1 Hybrids of Glycine soja and Glycine max
熊振宇 1李海波 1余爽1
作者信息
- 1. 武汉轻工大学生物与制药工程学院,湖北武汉 430000
- 折叠
摘要
[目的]探究野生大豆与栽培大豆之间遗传多态性的差异,以期为大豆杂交育种的亲本选配提供理论依据. [方法]用20对ISSR引物对野生大豆与栽培大豆及其10份杂种F1材料进行遗传多样性分析,探讨野生大豆与栽培大豆之间的遗传关系. [结果]此次试验共扩增出167条带,其中121条为多态性带,多态率达72.45 %;杂交F1代材料与其母本中黄19及父本ZYD04350间的平均遗传相似系数分别为0.65和0.47. [结论]栽培大豆与野生大豆杂交F1代材料间存在着丰富的变异,杂交F1代从母本获得的遗传物质多于父本.
Abstract
[Objective] To sudy the difference of genetic polymorphism between Glycine soja and Glycine max,so as to provide theory evidence for Glycine max crossbreeding.[Method] Twenty primers were used for ISSR analysis on Glycine soja,Glycine max and 10 F1 hybrids,to study the genetic relationship of Glycine soja and Glycine max.[Result] Total 167 bands were amplified in the PCR reactions.Among them 121 were polymorphic bands and the polymorphic rate was 72.45%.The average genetic coefficients between Zhonghuang 19 and F1 progenies,ZYD04350 and F1 progenies were 0.65 and 0.47,respectively.[Conclusion]There was abundant genetic variation among F1 progenies derived from crossing between Glycine soja and Glycine max,genetic material of F1 hybrids obtained from maternal was more than male parents.
关键词
野生大豆/栽培大豆/ISSR/多态性Key words
Glycine soja/Glycine max/ISSR/Polymorphism引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据