不同小干松种源的SRAP分析
SRAP Analysis of 15 Provenances of Pinus contorta
王骞春1
作者信息
- 1. 辽宁省林业科学研究院,辽宁沈阳 110032
- 折叠
摘要
[目的]研究小干松种源遗传多样性,为小干松引种、育种工作提供基础资料.[方法]利用SRAP荧光标记技术对引种的15个小干松种源进行SRAP分析.[结果]9对荧光标记的SRAP引物共产生1 075个条带,多态性位点953个,平均多态性为88.7%,每对引物扩增条带75~146条,平均每对引物扩增条带119条;通过UPGMA软件对15个种源进行遗传聚类分析,15个种源的相似系数在0.761 3~0.876 0,以遗传相似系数0.850 0为阈值可将15个种源分为4个群体,同一地理来源的种源有一定的遗传差异,但大体呈现按地理来源聚类的趋势.[结论]15个小干松种源具有较高的基因多态性,遗传多样性丰富,小干松变异类型与地理分布有关.
Abstract
[Objective] To study the gentic diversity of Pinus contorta provenance for laying the foundation for introduction of P.contorta breeding.[Method] Using SRAP fluorescence technique to analyze 15 provenances of P.contorta.[Result]9 SRAP primers for fluorescent labeling produced a total of 1 075 bands, 953 polymorphic loci, the average polymorphism was 88.7%.Each primer amplified bands were from 75 to 146, an average of 119 bands.The similarity coefficients of 15 provenances were 0.761 3-0.876 0 by UPGMA software.Taking the genetic similarity coefficient of 0.850 0 as threshold, 15 provenances were divided into 4 clusters, the provenances with the same geographic origin had some genetic differences, but generally showed a trend of clustering according to geographic origin.[Conclusion]15 provenances of P.contorta had high genetic diversity, abundant genetic diversity, the variation types were related to geographic distribution.
关键词
小干松/种源/SRAPKey words
Pinus contorta/Provenances/SRAP引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据