GnRHR在不同日龄建昌黑山羊睾丸、附睾中的分布与表达
Localization and Distribution of GnRHR in the Testis and Epididymis from Different Age of Jianchang Black Goat
侯真真 1张谊 2张明1
作者信息
- 1. 四川农业大学动物科技学院,四川温江611130
- 2. 西昌学院动物科技学院,四川西昌615000
- 折叠
摘要
[目的]定位并比较不同日龄建昌黑山羊睾丸及附睾中GnRHR的分布及表达情况.[方法]分别收集55、104、300、1 140日龄建昌黑山羊的睾丸、附睾头和附睾尾组织,然后进行脱水、包埋、切片后,采用免疫组化定位GnRHR在各个组织中的分布.[结果]GnRHR在建昌黑山羊睾丸、附睾头和附睾尾组织细胞中均有分布,在上皮下层、基质层中分布较多,其表达量在300日龄的附睾尾和1 140日龄的睾丸中达到较高水平.[结论] GnRHR在睾丸、附睾中均有表达,而且不同日龄阶段表达存在组织细胞特异性.
Abstract
[Objective] To localize and compare the distribution and expression level of GnRHR in the testis and epididymis from different age of Jianchang black goat.[Method]The testis and epididymis were collected at 55,104,104,1 140 d after animal birth,then dehydrated,embedded and cut.Distribution of GnRHR was orientated by immunohistochemical assay.[Result] GnRHR in the testis,epididymal heads and tails,especially in subepithelial layer and stroma layer,in which had a higher expression level than other tissue was expressed.GnRHR expression was the most in the epididymis at 300 d and in the testis at 1 140 d.[Conclusion] GnRHR is expressed in the testis and epididymis,and its expression level has tissue-specificity at different age.
关键词
促性腺激素释放激素受体/睾丸/附睾/建昌黑山羊Key words
Gonadotropin Releasing Hormone receptor/Testis/Epididymis/Jianchang black goat引用本文复制引用
基金项目
四川省科技厅项目(2014JY0021)
凉山州科技局项目(15NYCX0025)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据