猪轮状病毒与猪传染性胃肠炎病毒混合感染的实验室诊断
Laboratory Diagnosis of Mixed Infections of Porcine Rotavirus and Transmissible Gastroenteritis Virus
方振华 1沈振国 1孙倩 1丁利2
作者信息
- 1. 海南职业技术学院热带农业技术学院,海南海口 570216
- 2. 海南师范大学生命科学学院,海南海口 571158
- 折叠
摘要
[目的]对海南省某猪场腹泻病死仔猪病原进行实验室诊断.[方法]通过临床症状观察与病理剖检,结合RT-PCR方法和病毒的分离培养进行病原鉴定.[结果]经过RT-PCR扩增,获得了PRV和TGEV阳性目的片段;应用MA-104细胞与PK-15细胞分别进行PRV与TGEV的分离培养后,可见典型的细胞病变,经过RT-PCR进一步鉴定最终确诊为PRV和TEGV混合感染.[结论]通过采用紧急接种和保守治疗等综合防治措施,获得了较好的防控效果.
Abstract
[Objective] To make laboratory diagnosis of dead pigs from a hoggery of Hainan Province.[Method] By using the clinical examination,pathologic autopsy,RT-PCR and cell culture methods,the pathogen of dead pigs were separated and identified.[Result] The positive fragments of porcine rotavirus(PRV)and transmissible gastroenteritis virus (TGEV)were obtained by RT-PCR.After PRV and TGEV were isolated and cultured in MA-104 cells and PK-15 cells respectively,the typical cytopathic effect displayed.The mixed infection of PRV and TGEV was further identified by RT-PCR.[Conclusion] Better curative effects were obtained after comprehensive treatment measures such as emergent inoculation and conservative treatment were taken.
关键词
猪轮状病毒/猪传染性胃肠炎病毒/混合感染/实验室诊断Key words
Porcine rotavirus/Transmissible gastroenteritis virus/Mixed infections/Laboratory diagnosis引用本文复制引用
基金项目
海南省自然科学基金(20153086.20153081)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据