龙胆中黄酮化合物含量测定的方法学研究
Methodological Study of the Determination of Flavonoids from Radix Gentianae
朱丹 1杨菁 1李林龙 1杨航 1黎燕1
作者信息
- 1. 贵阳中医学院药学院,贵州贵阳 550025
- 折叠
摘要
[目的]建立紫外可见分光光度法测定龙胆中总黄酮含量的方法.[方法]通过微波辅助双水相提取龙胆中总黄酮类化合物,采用紫外可见分光光度法,以芦丁作为对照品,测定其含量.[结果]黄酮类化合物浓度在10.4~73.0 μg/mL,其吸光度与浓度有良好的线性关系;该方法的精密度、重复性和加样回收率的RSD值分别为0.30%、1.31%、2.27%.[结论]该方法准确、快速、重复性好,可以用于测定龙胆中总黄酮类化合物.
Abstract
[Objective] The research aimed to establish a method for determination of the total flavonoids from Radix Gentianae.[Method]The total flavonoids in Radix Gentianae were extracted by microwave-assisted aqueous two-phase.The contents of total flavonoids were determined by UV-visible spectrophotometry with rutin as the reference substance.[Result]The method was a good linear at the range of 10.4-73.0 μg/mL of the total flavonoids.The RSD values of the precision test, reproducibility experiment and the recovery rate test were 0.30%,1.31%,2.27%,respectively.[Conclusion]The method is accurate, rapid and reproducible,and can be used to determine the total flavonoids from Radix Gentianae.
关键词
龙胆/黄酮化合物/紫外可见分光光度法/含量测定/方法学Key words
Radix Gentianae/Flavonoids/UV-visible spectrophotometry/Determination of content/Methodology引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据