液相色谱串联质谱法测定动物组织中17种β-兴奋剂残留
Determination of 17 β-agonists Residual in Animal Tissue by Liquid Chromatography-Tandem Mass Spectrometer
招钰娟 1邓晓华 1甘沛明 1梁雪琪 1黎小鹏2
作者信息
- 1. 中山市农产品质量监督检验所,广东中山 528400
- 2. 中山市三乡镇农产品检验检测站,广东中山 528463
- 折叠
摘要
[目的]建立动物组织中β-兴奋剂多残留的液相色谱-串联质谱(LC-MS/MS)分析方法.[方法]样品经提取、固相萃取柱净化后采用LC-MS/MS 进行检测分析,对样品前处理条件和质谱参数进行研究.[结果]17种β-兴奋剂的线性范围在0.5~50.0 ng/mL相关性良好,线性相关系数在0.999 88~0.995 70;2种加标水平回收率为63.20%~109.92%和62.99%~112.19%,相对标准偏差(RSD)为0.63%~11.50%和0.33%~8.93%.[结论]该方法稳定、灵敏度高、选择性好,测定结果令人满意,可在各食品检测机构推广应用.
Abstract
[Objective] A sensitive and rapid HPLC-MS/MS method was developed for the determination of Beta-adrenergic agonistsresidues in animal tissue.[Method] Samples were subjected to extraction, followed by solid phase extraction clean-up before HPLC-MS/MS analysis.The pretreatment conditions and mass spectra parameters were studied.[Result] The developed standard curves of 17 Beta-adrenergic agonists all displayed good linearity over the concentration range of 0.5-50.0 ng/mL, and coefficient correlation (R2) between 0.999 88-0.995 70.Mean recovery rates of 6 replicates for Beta-adrenergic agonist in a negative pork sample spiked at two levels between 63.20%-109.92%;62.99%-112.19%, RSD was 0.63%-11.50% and 0.33%-8.93%.[Conclusion] This method proved to be of stability, high sensitivity and good selectivity and provided satisfying determination results.Therefore, its application deserves to be popularized among various food testing laboratories.
关键词
β-兴奋剂/动物组织/液相色谱串联质谱/残留Key words
β-agonists/Animal tissue/Liquid chromatography-tandem mass spectrometer/Residual引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据