地稔总黄酮含量的测定
Determination of Total Flavonoids from Melastoma dodecandrum Lour.
杨菁 1朱丹 1杜雨涵 1陈华巧 1于妮1
作者信息
摘要
[目的]建立地稔中总黄酮含量的测定方法.[方法]采用紫外-可见分光光度法,以芦丁为对照,以NaNO2-Al(NO3)3-NaOH为显色剂,检测波长为500 nm,测定地稔中总黄酮含量.[结果]芦丁浓度在0.01 ～0.07 mg/mL时与吸光度呈良好的线性关系(R2=0.9998);平均加样回收率为99.34％,相对标准偏差为0.56％.[结论]该方法简单可靠、精密度高、重现性好,为地稔的进一步开发利用提供了理论依据.
Abstract
[Objective] To establish a method for the total flavonoids content determination from Melastoma dodecandrum Lour.[Method] The content of total flavonoids from Melastoma dodecandrum Lour.was determined by UV-visble spectrophotometry with rutin as reference substance,sodium nitrite-aluminum nitrate and sodium hydroxide as chromogenic agent at the detection wavelength of 500 nm.[Result] The rutin showed a good linear relationship at the range of 0.01-0.07 mg/mL(R2 =0.999 8).The average recovery rate was 99.34％,and the relative standard deviation was 0.56％.[Conclusion] The method is simple and reliable,reasonable,specific,reproducible and provides experiment basis for the development of Melastoma dodecandrum Lour..
关键词
地稔/总黄酮/紫外-可见分光光度法Key words
Melastoma dodecandrum Lour./Total flavonoids/UV-visble spectrophotometry引用本文复制引用
基金项目
国家级大学生创新创业训练计划项目(责中医大创合字[2015] 12号)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据