‘火焰’品种彩色马蹄莲组培快繁体系的建立及优化
The Establishment and Optimization of Tissue Culture and Rapid Propagation System of Zantedeschia hybrida'Flame'
张慧 1罗珍珍 1由翠荣 2孔艳辉 1解晓旭 1孙印兵1
作者信息
- 1. 烟台市园林管理处科研所,山东烟台264000
- 2. 烟台大学生命科学学院,山东烟台264005
- 折叠
摘要
[目的]建立并优化‘火焰’品种彩色马蹄莲组培快繁体系.[方法]以‘火焰’品种彩色马蹄莲的球茎嫩芽为试验材料,对其组织培养无性繁殖体系进行研究.[结果]最佳诱导培养基为MS +2.0 mg/L 6-BA+ 0.2 mg/LNAA;最佳增殖培养基为MS +2.0 mg/L6-BA +0.2 mg/L NAA;植株再生最适合培养基为1/2MS +0.2 mg/L 6-BA+ 0.2 mg/L NAA;最适生根培养基为1/2MS+0.2 mg/LNAA +2.0 g/L活性炭.[结论]该研究建立了‘火焰’品种彩色马蹄莲的组培快繁体系,为其快速繁殖及产业化生产提供了技术和理论依据.
Abstract
[Objective]To establish and optimze the tissue culture and rapiad progration system of Zantedeschia hybrida'Flame'.[Method] The bulb shoots of Z.hybrida'Flame'were used as experimental materials to study the system of tissue culture and rapid propagation.[Result] The optimal medium for induction was MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA.The best proliferation medium was MS + 2.0 mg/L 6-BA + 0.2 mg/L NAA.The most suitable plant regeneration medium was 1/2MS + 0.2 mg/L 6-BA + 0.2 mg/L NAA.The optimum rooting medium was 1/2MS +0.2 mg/L NAA + 2.0 g/L activated carbon.[Conctustion] The tissue culture and rapid propagation system of Z.hybrida'Flame'was established to provide technology and the theoretical forndation for its rapid propagation and industrialized production.
关键词
‘火焰’彩色马蹄莲/组培快繁/优化Key words
Zantedeschia hybrida'Flame'/Tissue culture and rapid propagation/Optimization引用本文复制引用
基金项目
山东省科技发展计划项目(2013GNC11037)
烟台市科技发展计划项目(2015GNC113)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据