QuEChERS-液质联用法测定水果蔬菜中氯吡脲残留
Determination of Forechlorfenuron Residue in Fruits and Vegetables by QuEChERS Extraction and HPLC-MS/MS
胡江涛 1俞凌云 1帅培强 1于刚 1何开蓉1
作者信息
- 1. 四川出入境检验检疫局检验检疫技术中心,四川成都610041
- 折叠
摘要
[目的]采用快速提取-高效液相色谱-串联质谱法(QuEChERS-HPLC-MS/MS)建立水果蔬菜中氯吡脲残留的检测方法.[方法]样品经含0.1％乙酸的乙腈溶液提取,经QuEChERS法净化后采用HPLC-MS/MS法测定其氯吡脲残留量.[结果]该方法下氯吡脲在水果蔬菜中的检出限(LOD)为1.0 μg/kg,定量限(LOQ)为5.0 μg/kg.在2.0～100.0 μg/L范围内线性关系良好(R2 ＞0.999).在10.0～500.0 μg/kg的添加水平上,方法的平均回收率介于72.0％ ～ 115.0％,方法的相对标准偏差为1.5％ ～9.8％.[结论]该方法适用于水果蔬菜中氯吡脲的测定.
Abstract
[Objective] A quick extraction method QuEChERS-HPLC-MS/MS was established to determine forechlorfenuron in fruits and vegetables.[Method] Fruits and vegetables were extracted with 0.1％ acetic acid of acetonitrile solution and purified by QuEChERS,then forechlorfenuron residues were determined by HPLC-MS/MS.[Result] The limits of detection (LODs) and low determination limit (LOQ) for the forechlorfenuron was 1.0 μg/kg and 5.0 μg/kg in fruits and vegetables,respectively.Regression equations of these hormones had a good linear relationship (R2 ＞ 0.999) within 2.0-100.0 μg/L.The average recoveries of forechlorfenuron was in the range of 72.0％-115.0％with the coefficients of variation between 1.5％ and 9.8％ at the spiked levels of 10.0-500.0 μg/kg.[Conclusion] The method can be applied for the determination of the forechlorfenuron in fruits and vegetables.
关键词
检测/氯吡脲/水果蔬菜/高效液相色谱-串联质谱法Key words
Determination/Forechlorfenuron/Fruits and vegetables/HPLC-MS/MS引用本文复制引用
基金项目
四川省科技支撑计划项目(2015GZ0084)
四川省科技支撑计划项目(SK0604)
四川省卫生厅项目(2012-001)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据