凝胶渗透色谱-气相色谱法测定蔬菜中抑菌灵残留
Determination of Dichlofluanid Residue in Vegetables by Gel Permeation Chromatography and Gas Chromatography
高小丽 1曹淑瑞 2陈宇 2李贤良 2郗存显 2郑小玲 2张雷 2王国民2
作者信息
- 1. 重庆化工职业学院,重庆401228
- 2. 重庆出入境检验检疫局,重庆400020
- 折叠
摘要
[目的]建立凝胶渗透色谱(GPC)-气相色谱法测定蔬菜中抑菌灵残留量的方法.[方法]样品用环己烷-乙酸乙酯(1:1,V/V)提取,GPC净化,气相色谱-电子捕获检测器(GC-ECD)检测.[结果]抑菌灵在0.01～1.00 mg/L时线性关系良好(R2 =0.999 3),方法检测限(S/N=3)为0.32 μg/kg.在0.01、0.02、0.04 mg/kg添加水平下,加标回收率为74.7％ ～95.7％,相对标准偏差(n=6)为0.5％ ～3.5％.[结论]该方法适用于蔬菜中抑菌灵残留量的测定.
Abstract
[Objective] A method for determination of dichlofluanid residue in vegetables by gel permeation chromatography and gas chromatopraphy was developed.[Method] Samples were extracted with cyclohexane-ethyl acetate (1∶ 1,V/V),and then detected by GC-ECD after being cleaned up with GPC.[Result] It turned out that dichlofluanid showed a good linearity over the range of 0.01-1.00 mg/L with correlation coefficient of 0.999 3.The limit of detection (LOD) was 0.32 μg/kg.The recoveries of the dichlofluanid were in the range of 74.7％-95.7％ with the spiked levels of 0.01,0.02 and 0.04 mg/kg,and the relative standard deviations (RSDs) were in the range of 0.5％-3.5％.[Conclusion] The method is suitable for detecting dichlofluanid residue in many kinds of vegetables.
关键词
凝胶渗透色谱-气相色谱法/抑菌灵/蔬菜/残留Key words
Gel permeation chromatography-gas chromatography/Dichlofluanid/Vegetable/Residue引用本文复制引用
基金项目
重庆市青年科技人才培养计划项目(cstc2014kjrc-qnrc00002)
国家质量监督检验检疫总局科技计划项目(2016IK296)
重庆市应用开发重点项目(cstc201yykfB0165)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据