基于纤维降解能力分析的青贮饲料发酵用灵芝菌株的筛选
Screening of Ganoderma lucidum for Silage by Degradation Ability Analysis of Cellulase and Ligninase
丁琳 1薛绿艳 1赵雅敏 1钱蕾 1李佳华 1卢晓凤 1曹军1
作者信息
摘要
[目的]获得较适宜秸秆降解的灵芝菌株.[方法]通过显色圈法初筛和比色法复筛从28株食品级灵芝菌种中筛选适宜秸秆发酵的灵芝菌株的方法.[结果]添加黑芝进行青贮饲料的辅助发酵,经过1cd即可进入乳酸发酵阶段,45d即可获得pH小于4.2的青贮饲料,且具有较高的纤维素酶和木质素酶活力的菌株发酵所得的饲料中乳酸含量更高.[结论]添加灵芝可以加速乳酸发酵的起始时间,并能缩短青贮饲料发酵的周期,提高饲料中的乳酸含量.
Abstract
[Objective] To screen out Ganoderma lucidum strain for straw degradation.[Method] Ganoderma lucidum strain for straw fermentation was screened out from 28 strains of food-level strains of G.lucidum by color circle method and colorimetric screening.[Result] Ganoderma neo-japonicum was added to assist the fermentation of silage,it entered the fermentation stage of lactic acid after one day.After 45 days,silage with pH of less than 4.2 was obtained.The feed fermented with strain with higher activities of cellulase and ligninase had high content of lactic acid.[Conclusion] Adding G.lucidum can accelerate the start time of lactic acid fermentation,shorten the cycle of silage fermentation,and increase the content of lactic acid in feed.
关键词
灵芝/青贮饲料/纤维降解/乳酸发酵Key words
Ganoderma lucidum/Silage/Fiber degradation/Lactic acid fermentation引用本文复制引用
基金项目
南通大学大学生创新训练计划项目(2016061)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据