椰子SSR分子标记筛选
Screening of SSR Markers for Coconut
吴翼 1李静 1杨耀东1
作者信息
- 1. 中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌571339
- 折叠
摘要
[目的]筛选可用的SSR分子标记,加快椰子分子辅助育种进程.[方法]采用改良CTAB法提取椰子基因组DNA,以基因组DNA为模板,对36对SSR引物进行筛选,以条带清晰、多态性丰富为引物筛选原则.[结果]共筛选出7对具有多态性的引物,其中2对多态性较丰富,可用于后续SSR分子标记分析.[结论]该研究为开展椰子种质资源遗传多样性系统分析提供了SSR引物.
Abstract
[Objective] To accelerate the molecular assisted breeding process of coconut by screening SSR markers.[Method] The genomic DNA was extracted by modified CTAB method,and 36 SSR primers were screened with genomic DNA as template.[Result] A total of 7 pairs of polymorphic primers were screened,of which more than 2 pairs of polymorphism were found,which could be used for subsequent SSR molecular marker analysis.[Conclusion]The study provides the SSR primers for analysis of coconut germplasm genetic diversity system.
关键词
椰子/SSR/DNA提取/聚丙烯酰胺凝胶电泳Key words
Coconut/SSR/DNA extraction/PAGE Gel引用本文复制引用
基金项目
海南省国际合作专项(KJHZ2014-24)
农业部热带作物种质资源保护项目(16RZZY-104)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据