抗性家蚕血液中与BmNPV抗性相关铜化合物的分离方法研究
Study on the Separation Methods of Copper Compounds Related to BmNPV Resistance in Hemolymph of Bombyx mori
李龙 1孙慧 1刘泽玉 1徐安英 2钱荷英 2武国华1
作者信息
- 1. 江苏科技大学蚕业研究所,江苏镇江 212018;农业部蚕桑产品及食用昆虫质量安全风险评估实验室,江苏镇江 212018
- 2. 农业部蚕桑产品及食用昆虫质量安全风险评估实验室,江苏镇江 212018
- 折叠
摘要
[目的]探究家蚕血淋巴中与BmNPV抗性相关铜化合物的分离方法.[方法]选取对 BmNPV 具有抗性的家蚕品种和非抗性品种为研究对象,对它们进行添食 BmNPV 病毒处理.提取家蚕的血淋巴,运用SEC-ICP-MS对2组样品中Cu化合物进行分离和检测.[结果]筛选出优化的体积排阻色谱分离条件:YarraTM SEC-2000分离柱,30 mmol/L Tris-醋酸为流动相(pH 7.4),流速0.5 mL/min.建立了分离与检测家蚕血液中Cu化合物的SEC-ICP-MS联用技术.BmNPV抗性和非抗性家蚕血液中Cu化合物主要的差异是位于7.6 min的组分.[结论]制备了葡聚糖G100凝胶柱分离并收集可能与BmNPV 抗性相关的家蚕血液分离液,为家蚕金属蛋白的分离提供更简便有效的方法.
Abstract
[Objective]To study the separation methods of copper compounds related to BmNPV resistance in hemolymph of Bombyx mori.[Method]B.mori cultivars with and without BmNPV resistance were selected as research objects to feed BmNPV virus.Cu compounds was extracting and determinated from hemolymph of two groups B.mori by SEC-ICP-MS.[Result]The optimal seperation conditions for size exclusion chromatography were as follows:YarraTM SEC-2000,30 mmol/L Tris-acetic acid(pH 7.4) as mobile phase, flow rate of 0.5 mL/min.SEC-ICP-MS technology for separating and detecting copper compounds in hemolymph of B.mori was established.The main differences of copper compounds between resistant cultivars of B.mori and non-resistant cultivars of B.mori was the compounds with the retention time of 7.6 min.[Conclusion]The separation liquid of hemolymph of Bombyx mori might be related with BmNPV resistance seperated and collected by glucan G100 gel column was prepared.The research can provide simpler and more effective methods for the seperation of metal proteins in B.mori.
关键词
家蚕/BmNPV/抗性/体积排阻色谱/电感耦合等离子体质谱Key words
Bombyx mori/BmNPV/Resistance/Size exclusion chromatography/ICP-MS引用本文复制引用
基金项目
江苏特聘教授项目(苏教师〔2015〕17)
2017年农业部农产品风险评估项目()
"十二五"现代农业产业技术体系建设专项(CARS-18)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据