正交法优选亮菌多糖脱色工艺研究
Study on Decoloration Technique of Armillariella Polysaccharide by Orthogonal Experiments
黄纯 1王小平 1程海涛 1姜晟1
作者信息
摘要
[目的]建立亮菌多糖脱色的最佳方法.[方法]以色素脱除率作为主要考察指标,通过单因素考察和正交法优化亮菌多糖脱色工艺.[结果]D152树脂、45 ℃、pH 5.0为最佳脱色条件.[结论]D152作用后色素脱除率高,糖保留率高,有益于下一步分离纯化.
Abstract
[Objective] The research aimed to establish the optimum method of decoloration about Armillariella polysaccharide.[Method]Taking the decoloring ratio as main index,the optimum decoloring technique were established through single factorial test and orthogonal experiments.[Result]The best method of decoloration were obtained as follows: D152 resins working in pH 5.0 and 45 ℃.[Conclusion]The decoloring ratio of D152 and the reteion rate of polysaccharide are high,which is beneficial to the next step.
关键词
脱色素/亮菌多糖/工艺优化/正交法Key words
Decoloration/Armillariella polysaccharide/Technology optimization/Orthogonal method引用本文复制引用
出版年
2017