聚乙烯醇降解菌HK1菌株鉴定与降解特性
Identification and Degradation Character of a Polyvinyl Alcohol Degradable Bacteria HK1
刘云柯 1周云横 1陈少林 1卫亚红1
作者信息
- 1. 西北农林科技大学生命科学学院,陕西杨凌 712100;西北农林科技大学旱区生物质能研究中心,陕西杨凌 712100
- 折叠
摘要
[目的]鉴定聚乙烯醇降解菌HK1并研究其降解特性.[方法]以一株能以聚乙烯醇为唯一碳源生长的细菌HK1为出发菌株,通过形态观察和生理生化试验对其进行鉴定,研究其对几种实验室常用抗生素的抗性,并通过摇瓶试验研究其降解特性.[结果]经鉴定,该菌确定为解淀粉芽孢杆菌属细菌,30 ℃、180 r/min摇床培养后,对培养基中PVA(1 g/L)的降解率达36.26%.HK1降解PVA的环境最优条件如下:温度为30 ℃,底物浓度为1 g/L,pH 8.0,酵母粉添加量为2 g/L.[结论]研究结果为系统研究该菌株提供了参考.
Abstract
[Objective] The aim was to identify a polyvinyl alcohol degradable bacteria HK1 and study its degradation character.[Method] We took a strain HK1 which could grow with polyvinyl alcohol as the only carbon source,identified it through its morphology and physiochemical characteristics,studied the resistance to several kinds of antibiotics which were commonly used in the laboratory,and studied its degradation character through shake flask test.[Result] According to its morphology,physiochemical characteristics and 16S rDNA sequence analysis,HK1 was identified as Bacillus amyloliquefaciens.It could degrade 36.26% of PVA (1 g/L) after culturing at 30 ℃,180 r/min in shaking flasks.The optimal conditions for PVA biodegradation by the obtained strain were as followed:30 ℃,1 g/L PVA,pH 8.0,and 2 g/L yeast.[Conclusion] The results can provide reference for further research of the strain.
关键词
聚乙烯醇/解淀粉芽孢杆菌/菌株鉴定/生物降解Key words
Polyvinyl alcohol/Bacillus amyloliquefaciens/Identification/Biodegradation引用本文复制引用
基金项目
杨凌示范区科技计划项目(2015NY-07)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据