高粱液体再生系统初步研究
Preliminary Study on Sorghum Liquid Regeneration System
王良群 1刘勇 2郝艳芳 1张微 1杨伟 2白鸿雁 2武擘2
作者信息
- 1. 山西省农业科学院高粱研究所,山西晋中 030600;高粱遗传与种质创新山西省重点实验室,山西晋中 030600
- 2. 山西省农业科学院高粱研究所,山西晋中 030600
- 折叠
摘要
选用幼穗为外植体并进行匀浆,将匀浆物进行振荡培养,获得颗粒状培养物.再把这些颗粒状培养物接入到液体分化培养基中,振荡培养后有些颗粒会萌发出新芽,然后把这些已长出芽的培养物接入到固体分化培养基中可获得完整的苗.研究还将幼穗固体培养模式获取的愈伤组织接入到液体分化培养基中进行振荡培养,结果获取了相互分离能生根发芽的胚状体.该研究丰富了高粱再生培养技术手段.
Abstract
The immature inflorescences was used as explant and homogenized.The homogenate was cultured with shaking.The granular callus-like culture was obtained and accessed into liquid differentiation edium.After further culturing,germination of sprouts will occur on some granules and then these geminated granules were added into solid regeneration culture medium.Lastly,complete seedlings can be obtained.In the study,the callus of the immature inflorescences was cultured in the liquid differentiation medium.As a result,the embryoid bodies isolated from each other were obtained.The sorghum liquid regeneration culture was explored to enrich the sorghum tissue culture technology.
关键词
高粱/液体再生系统/静止培养/振荡培养Key words
Sorghum/Liquid regeneration system/Static culture/Shaking culture引用本文复制引用
基金项目
山西省科技厅攻关项目(20150311003-2)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据