鸭源新城疫病毒的分离与鉴定
Separation and Identification of Duck-origin Newcastle Disease Virus
胡晓苗 1戴银 1张丹俊 1赵瑞宏 1潘孝成 1周学利 1侯宏艳 1沈学怀 1朱传明1
作者信息
- 1. 安徽省农业科学院畜牧兽医研究所,安徽合肥 230031
- 折叠
摘要
[目的]分离并鉴定鸭源新城疫病毒.[方法]从安徽地区鸭病料中分离新城疫病毒,测定其鸡胚半数感染量(EID50)、鸡胚平均致死时间(MDT)和1日龄雏鸡脑内接种致病指数(ICPI).[结果]2株新城疫病毒均为强毒.通过RT-PCR技术对这2株鸭新城疫病毒的F基因进行序列测定与分析,结果发现F基因裂解位点为112RRQKRF117,符合NDV强毒株裂解位点氨基酸序列.F基因遗传进化树分析发现,AH1株属于基因Ⅶ型,而AH2株属于基因Ⅵ型,来源于鸽源病毒.[结论]水禽新城疫病毒(NDV)的感染来源复杂,应避免将不同种类的家禽混合饲养.
Abstract
[Objective] To separate and identify newcastle disease virus (NDV) from duck.[Method] NDV were separated from diseased duck samples from Anhui Province and their EID50,MDT and ICPI were determined.[Result] Two strains of NDV were virulent.F gene of two strains of NDV were sequenced by RT-PCR,and the results showed that the cleavage site of F gene of the viruses was 112RRQKRF117,which was accorded with the amino acid sequences of cleavage site of highly-pathogenic strain of NDV.The genetic evolutionary tree analysis of F gene showed that AH1 belonged to genotype Ⅶ,but AH2 belonged to genotype Ⅵ,which was from pigeon sources.[Conclusion] The infection sources of NDV from water birds were complex,so the mixed breeding of different varieties of fowls should be avoided.
关键词
鸭/新城疫病毒/分离/鉴定/F基因Key words
Duck/NDV/Separation/Identification/F gene引用本文复制引用
基金项目
安徽农业科学院学科建设项目(16A0411)
国家现代农业产业技术体系项目(CARS-41)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据