中草药白头翁中总黄酮提取工艺研究
Study on Technology of Extracting Total Flavonoids from Radix Pulsatillae
雒江菡 1于瑞洪2
作者信息
- 1. 哈尔滨商业大学,细胞与分子生物学研究所,黑龙江哈尔滨 150076
- 2. 黑龙江生物科技职业学院,黑龙江哈尔滨 150025
- 折叠
摘要
[目的]研究中草药白头翁中总黄酮的提取工艺.[方法]以白头翁为原料提取总黄酮,通过对影响白头翁中总黄酮得率的4种因素(料液比、提取温度、提取时间和乙醇浓度)进行单因素试验,确定白头翁中提取总黄酮的最佳工艺.[结果]白头翁中提取总黄酮的最佳条件为:料液比为1:20,提取温度为70 ℃,提取时间为3 h,乙醇浓度为70%,此时,总黄酮得率为8.29%.[结论]该研究为建立白头翁黄酮的质量标准、有效地控制白头翁药材的质量奠定基础.
Abstract
[Objective] The research aimed to study the technology of extracting total flavonoids from Radix Pulsatillae.[Method]Using Radix Pulsatillae as raw material to extract total flavonoids,the optimum extraction technology of total flavonoids from Radix Pulsatillae was determined by single factor test of four factors (solid-liquid ratio,extraction temperature,extraction time and ethanol concentration) affecting the total flavonoid yield.[Result]The total flavonoid yield was 8.29% when the optimum extraction conditions were as follows:1:20 solid-liquid ratio,extraction temperature of 70 ℃,extraction time of 3 h,ethanol concentration of 70%.[Conclusion]This study laid the foundation for the establishment of quality standards for Radix Pulsatillae flavonoids and effective control of the quality of Radix Pulsatillae.
关键词
白头翁/总黄酮/单因素试验/提取工艺Key words
Radix Pulsatillae/Total flavonoids/Single factor test/Extraction technology引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据