软枣猕猴桃"寒玉1号"组培技术研究
Tissue Culture Technique for Actinidia arguta "Hanyu No.1"
杜鹏瑶 1刘德江 1徐彪 1赵佳明 1申健1
作者信息
- 1. 佳木斯大学生命科学学院,黑龙江佳木斯 154007
- 折叠
摘要
[目的]探索软枣猕猴桃"寒玉1号"适宜组培方法.[方法]以新生腋芽为外植体,筛选最佳消毒方法,并对影响其生长的NAA、6-BA 等生长调节物质的浓度进行筛选.[结果]以5% NaClO 8 min+0.1% HgCl2 6 min或9 min,5% NaClO 10 min+0.1% HgCl2 6 min为适宜消毒方法,污染率和褐化率均较低;腋芽在WPM+6-BA 3.0 mg/L+NAA 0.2 mg/L培养基中增殖效果最佳,增殖系数可达5.6,株高3.9 cm;丛生芽在WPM+NAA 0.3 mg/L培养基中诱导生根效果最佳,生根率可达100%,平均根条数为4.4条,平均根长为5.2 cm.[结论]软枣猕猴桃"寒玉1号"可采用组织培养的方法进行快速繁殖.
Abstract
[Objective] To explore the suitable tissue culture technique for Actinidia arguta "Hanyu No.1".[Method] With axillary bud as explant,the best disinfection method was filtered,and the NAA,6-BA concentration were selected.[Result] The best disinfection methods were 5% NaClO 8 min+0.1%HgCl2 6 min or 9 min,5% NaClO 10 min+0.1%HgCl2 6 min,in which the mortality rate and contamination rate were lower.The best medium of inducing germinating of explant was WPM+6-BA 3.0 mg/L +NAA 0.2 mg/L,in which the ratio of proliferation was 5.6 and the height of plant was 3.9 cm.The best medium of inducing rooting from cluster buds was WPM+NAA 0.3 mg/L,in which the rate of rooting was 100%,the average number of root was 4.4 and the average length of root was 5.2 cm.[Conclusion] It concluded that Actinidia arguta "Hanyu No.1" can adopt the method of tissue culture for rapid propagation.
关键词
软枣猕猴桃/"寒玉1号"/腋芽/组织培养Key words
Actinidia arguta/"Hanyu No.1"/Axillary bud/Tissue culture引用本文复制引用
基金项目
佳木斯大学应用技术类项目(yz2014-005)
佳木斯大学大学生创新项目(2014xj15)
出版年
2017
国家科技期刊平台
NSTL
万方数据