Folin-Ciocalteu比色法测定头花蓼多酚含量
Determination of Polyphenols in Polygonum capitatum by Folin-Ciocalteu Colorimetry
江楠 1李潇彬 2顾曼琦 2廖莉玲2
作者信息
- 1. 贵州轻工职业技术学院,贵州贵阳550025
- 2. 贵州师范大学化学与材料科学学院,贵州贵阳550001
- 折叠
摘要
[目的]建立头花蓼中多酚含量的比色测定方法.[方法]以没食子酸为对照品,对测定头花蓼中多酚含量的比色条件(包括检测波长、Na2CO3用量、Folin-Ciocalteu试剂用量、反应温度及时间对吸光值的影响)进行了研究.[结果]当FC试剂用量为2.5mL,12％Na2CO3用量为7 mL时,在35℃下反应40 min为最佳反应条件;多酚的含量与760 nm处吸光度呈良好的线性关系;测定方法的平均回收率为98.66％,RSD为0.380％.[结论]该方法不仅操作简单,而且稳定性、重现性、精密度好,能够用于头花蓼提取液多酚含量的测定.
Abstract
[Objective] The research aimed to establish a colorimetric method for the determination of polyphenols in Polygonum capitatum.[Method]To gallic acid as a reference substance,the colorimetric conditions (the effects of detection wavelength,Na2CO3 dosage,Folin-Ciocalteu reagent dosage,reaction temperature and time on the absorbance value) were studied.[Result] When Folin-Ciocalteu reagent was 2.5 mL and 12％ sodium carbonate solution was 7 mL,reaction 40 min at 35 ℃,there was a good linearity between the absorbance and gallic acid concentration in 760 nm,the average recovery was 98.66％ and RSD was 0.380％.[Conclusion] This method is simple,accurate,repeatable and stable.It can be used for the determination of polyphenol content in Polygonum capitatum.
关键词
头花蓼/多酚/Folin-Ciocaheu比色法/检测条件Key words
Polygonum capitatum/Polyphenol/Folin-Ciocaileu colorimetry/Detecting condition引用本文复制引用
基金项目
贵州省科技厅_2014年度贵州省科技厅与贵州师范大学联合基金计划项目(黔科合LH字[2014]7064号)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据