牛乳中产色葡萄球菌的分离与鉴定
Isolation and Identification of Staphylococcus chromogenes in Milk
费育超 1倪宏波1
作者信息
- 1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆163319
- 折叠
摘要
[目的]确定送检的患乳房炎病牛乳汁中是否有产色葡萄球菌感染.[方法]从患乳房炎病牛乳汁中分离致病菌,并对其进行鉴定,最后通过药敏试验筛选出对其敏感的药物.[结果]分离菌16S rRNA基因片段为长度1422 bp的核苷酸序列.经BLAST分析可知,其与产色葡萄球菌16S rRNA核苷酸序列相似性高达99%,确定其为产色葡萄球菌.药敏试验结果表明,产色葡萄球菌对环丙沙星和四环素高度敏感,而对多黏菌素-B、青霉素、克林霉素和诺氟沙星等不敏感.[结论]研究结果可为确定乳房炎的病原及治疗方法提供新思路.
Abstract
[Objective]To check whether there was Staphylococcus chromogenes infection in the milk of diseased cattle with mastitis.[Method] Pathogen were isolated and identified from the milk of diseased cattle with mastitis.And the sensitive drugs were screened out by drug susceptibili-ty test.[Result] 16S rRNA gene fragment of the isolated bacteria was a nucleotide sequence with the length of 1422 bp.BLAST analysis showed that its similarity with Staphylococcus chromogenes 16S rRNA nucleotide sequence was up to 99%,so it was confirmed as Staphylococcus chromo-genes.Drug sensitivity test showed that it was highly sensitive to ciprofloxacin and tetracycline,and it was tolerant to polymyxin-B,penicillin,clin-damycin and norfloxacin and others.[ Conclusion] The research results can provide new ideas for studying the pathogen and treatment methods of mastitis.
关键词
奶牛乳房炎/16SrRNA/产色葡萄球菌/耐药性分析Key words
Cow mastitis/16S rRNA/Staphylococcus chromogenes/Drug tolerance analysis引用本文复制引用
基金项目
黑龙江八一农垦大学博士科研启动基金(校启D-2007-1)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据