2个育性恢复基因Rf1a和Rf1b在红莲型水稻中的表达量分析
Expression Analysis of Two Fertility Restorer Genes Rf1a and Rf1b in Honglian Rice
刘海青 1杨梦醒 1姜慧 1谭艳平 1刘学群1
作者信息
- 1. 中南民族大学生命科学学院,中南民族大学生物技术国家民委重点实验室武陵山区特色资源植物种质保护与利用湖北省重点实验室,湖北武汉430074
- 折叠
摘要
[目的]探究红莲型水稻细胞质雄性不育(CMS)与不同恢复基因之间的作用模式.[方法]利用特异性引物结合荧光定量PCR技术,在红莲型水稻近等恢复基因系L-Rf5、L-Rf6,恢复系9311和不育系粤泰A(YTA)中检测2个包台型恢复基因Rf1a和Rf1b的表达量.[结果]Rf1a在L-Rf5、L-Rf6、9311中都能表达,而Rf1b只能在部分材料或部分组织中表达.对同一时期二者的表达量分析发现,L-Rf5和9311的相同组织中Rf1a表达水平显著高于Rf1b,L-Rf6叶片中Rf1a的表达量则低于Rf1b.[结论]该研究结果对研究红莲型恢复系的遗传多样性具有一定的参考价值.
Abstract
[Objective]To investigate the mode of action of cytoplasmic male sterility (CMS) and different restorer genes in Honglian type rice.[Method]The expression of Rf1a and Rf1b of Boro-Ⅱ were tested by fluorescence quantitative PCR in the L-Rf5, L-Rf6, the restorer line 9311 and the sterile line YuetaiA(YTA).[Result]Rf1a can be expressed in all of them and Rf1b can only be expressed in partial of them. The expression level of Rf1a was significantly higher than Rf1b in L-Rf5 and 9311, and the expression of Rf1a in L-Rf6 was lower than that in Rf1b.[Conclusion]The results of this study have a certain value for the study of genetic diversity of the restorer gene in HL-type.
关键词
Rf1a/Rf1b/细胞质雄性不育/荧光定量PCRKey words
Rf1a/Rf1b/Cytoplasmic male sterility/Fluorescence quantitative PCR引用本文复制引用
基金项目
湖北省自然科学基金重点项目(2014CFA108)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据