红发夫酵母有机硒最佳转化条件的研究
Optimum Transformation Conditions of Organic Selenium by Phaffia rhdoozyma
徐洲 1朱文优 1魏琴 1黎维 1张超1
作者信息
- 1. 宜宾学院生命科学与食品工程学院,固态发酵资源利用四川省重点实验室, 四川宜宾644000
- 折叠
摘要
[目的]优化红发夫酵母(Phaffia rhodozyma)有机硒转化条件.[方法]通过正交试验研究硒添加量、培养时间和装液量对红发夫酵母富硒的影响.[结果]红发夫酵母富硒的最适培养基为PDA培养基,亚硒酸钠的添加方式为分2次添加,在硒添加量20 mg/L、培养时间30 h、装液量80 mL/500 mL的条件下,红发夫酵母的生物量达10.62 g/L,有机硒转化率达63.2%.[结论]研究结果为红发夫酵母富硒的进一步研究提供了参考.
Abstract
[Objective] The aim was to optimize the transformation conditions of organic selenium by Phaffia rhodozyma.[Method] The effect of selenium addition, culture time and liquid loading on selenium accumulation in Phaffia rhodozyma was studied by orthogonal experiment .[ Re-sult ] The optimum medium for selenium enrichment was PDA medium , and the adding method was added in two times .Under the condition of se-lenium content at 20 mg/L and the liquid loading at 80 mL/500 mL incubating for 30 h, the biomass and transformation rate of organic selenium could reach 106.2 g/L and 63.2%, respectively.[Conclusion] The results can be regarded as the reference for further research on selenium en -richment in Phaffia rhodozyma.
关键词
红发夫酵母/有机硒/转化添加/生物量Key words
Phaffia rhodozyma/Organic selenium/Conversion conditions/Biomass引用本文复制引用
基金项目
四川省重点实验室项目(NJ2014-10)
四川省重点实验室项目(2015GTY009)
四川省重点实验室项目(04JY029-102)
四川省教育厅/实验室项目(09ZC051)
四川省教育厅/实验室项目(2009KFJ001)
四川省高校科研创新团队建设资助项目(14TD0031)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据