西藏山羊DQA2基因多态性与血液免疫性状的相关性分析
Correlation Analysis between Polymorphisms of DQA2 Gene and Blood Immune Traits in Tibetan Goats
吕雪峰 1李亚东 2何茜 1高维明1
作者信息
- 1. 新疆畜牧科学院畜牧业质量标准研究所,新疆乌鲁木齐830046
- 2. 新疆师范大学,新疆乌鲁木齐830054
- 折叠
摘要
[目的]了解西藏山羊DQA2基因与血液免疫性状的关系.[方法]利用PCR-RFLP对西藏山羊DQA2基因外显子2多态性及其血液免疫指标效应进行了分析.[结果]在西藏山羊中共检测到4种基因型,由3个等位基因控制.其中,BB基因型淋巴细胞数目显著高于AB型和BC型(P<0.05),BB型中间细胞数目高于AB型和BC型,BB型平均血红蛋白含量低于AB型和BC型,但均差异不显著.[结论]西藏山羊BB基因型是影响血液淋巴细胞总数的重要因素,与血液免疫指标有一定的相关性,可作为抗病育种的遗传标记,也为后期的辅助选择提供了科学依据.
Abstract
[Objective] To understand the relationship between DQA2 gene and blood immune traits in Tibet goat.[Method]The polymorphism of exon 2 of DQA2 gene of Tibetan goat and the effects on blood immune traits were studied by PCR-RFLP.[ Result] Four genotypes were detected in Tibetan goat,which were controlled by three alleles.Lymphocyte count(W-SCC)with genotype BB was significantly higher than that with genotype AB and BC (P<0.05).Intermediate cell count (MID) with genotype BB was higher than that with genotype AB and BC.Mean corpuscular he-moglobin(MCH) with genotype BB was lower than that with genotype AB and BC,but there was no significant difference.[Conclusion]Genotype BB was an important factor affecting W-SCC.There was correlation between genotypes and blood immune traits,so it could be used as a genetic marker for disease resistance breeding,and it also provided scientific basis for the latter selective breeding.
关键词
西藏山羊/MHC-DQA2/血液免疫/多态性Key words
Tibetan goats/MHC-DQA2/Blood immune/Polymorphism引用本文复制引用
基金项目
新疆维吾尔自治区自然科学基金(2016D01A004)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据