桑黄扩大培养过程中黄酮产量变化研究
Study on the Change of Flavonoids Production in the Expanding Culture Process of Phellinus igniarius
杜睿绮 1王芯蕾 2许谦2
作者信息
- 1. 菏泽一中,2015级宏志级部,山东菏泽274000
- 2. 菏泽学院农业与生物工程学院生命科学系,山东菏泽274000
- 折叠
摘要
[目的]对桑黄进行液体培养基的扩大培养,分析扩大培养过程中黄酮产量的变化.[方法]250、500、1000 mL 3种锥形瓶分别装液体培养基150、300、600 mL,在28℃、180 r/min条件下培养.采用有机溶剂浸提法提取黄酮,分光光度法测定黄酮产量.[结果]在扩大培养过程中,桑黄胞内和胞外黄酮产量均呈现先下降再上升的趋势.[结论]该研究结果对桑黄胞内和胞外黄酮的生产、提取及应用具有现实的指导意义.
Abstract
[Objective] The research aimed to expand the culture medium of Phellinus igniarius and analyze the change of flavonoid production during the culture process.[Method]150, 300, 600 mL liquid medium were stalled in 250, 500, 1000 mL flasks respectively and incubated under 28 ℃, 180 r/min conditions.Organic solvent extraction was used to extract flavonoids and spectrophotometry was used to measure fla-vonoids production.[Result]In the process of expanding the culture, the intracellular and extracellular flavonoids production of Phellinus igni-arius showed a tendency to decrease first and then rise.[Conclusion] The results of this study have guiding significance for the production, ex-traction and application of intracellular and extracellular flavonoids from Phellinus igniarius.
关键词
桑黄/液体培养基/扩大培养/黄酮产量Key words
Phellinus igniarius/Liquid culture medium/Expanded culture/Flavonoid production引用本文复制引用
基金项目
山东省重点研发项目(2016GNC113019)
菏泽市科技发展计划项目(2012N002)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据