摘要
[目的]使用多种培养成分组合,筛选出一个适合山羊诱导性多能干细胞(induced pluripotent stem cells,iPSCs)的培养体系.[方法]采用慢病毒作为载体,携带多能因子,采用多种培养成分(培养基、血清和细胞因子)的组合培养山羊iPSCs,优化其培养体系.[结果]用FBS代替KSR可以提高克隆的形成效率以及克隆的质量,而用DMEM/F12代替KNOCKOUT-DMEM,iPSCs克隆的形成效率和克隆质量没有明显的改善.带有β-FGF的培养体系可以保持iPSCs未分化的状态,而培养体系中只加LIF不能维持iPSCs未分化的状态.[结论]该研究成功诱导出山羊iPSCs,且能在体外传代培养.
Abstract
[Objective]A culture system suitable for goat-induced pluripotent stem cells (iPSCs) was screened by using combination of various culture components.[Method]With the lentivirus as vector carrying pluripotency factor,goat iPSCs were cultured with a variety of culture compo-nents ( culture medium,serum and cytokine) to optimize the culture system.[ Result] The forming efficiency and clone quality of clones could be improved by FBS instead of KSR.The forming efficiency and clone quality of iPSCs clones were not improved by DMEM /F12 instead of KNOCK-OUT-DMEM.The culture system withβ-FGF could maintain the undifferentiated state of iPSCs,while only LIF could only maintain the undifferen-tiated state of iPSCs in the culture system.[Conclusion]Goat iPSCs were successfully induced and cultured in vitro.
国家科技期刊平台
NSTL
维普
万方数据