高效液相色谱法测定海洋生物中牛磺酸的含量
Detection of Taurine in Marine Life by High Performance Liquid Chromatography
柳小英 1毛丽沙 1鲍敏洁 1周瑾 1金火喜1
作者信息
- 1. 浙江海洋大学食品与医药学院,浙江舟山316022
- 折叠
摘要
[目的]建立牛磺酸的高效液相色谱分析方法,并通过该方法测定不同海洋生物中牛磺酸的含量.[方法]应用邻苯二甲醛(OPA)柱前衍生法,研究流动相比例、流速、衍生剂比例、衍生时间等因素对牛磺酸检测的影响.[结果]当牛磺酸∶OPA=1∶2,衍生时间2 min,流动相乙腈∶水=15∶85,流速为0.5 mL/min,检测波长为360 nm时,牛磺酸浓度为10～400 μg/mL时具有良好的线性关系(R2＞0.99),平行样品的相对标准偏差为2.9％ ～7.4％.利用该方法检测不同海产品中牛磺酸的含量,所选择的海洋贝类、甲壳类、鱼类中均含有牛磺酸,其中海瓜子中含量最高,达11.2 mg/g.不同组织比较,鱼肉中牛磺酸含量较高,而鳃中较低.[结论]该研究结果丰富了海洋生物中牛磺酸含量的数据,为进一步研究牛磺酸在不同组织中的合成机制提供了参考.
Abstract
[Objective] The aim was to establish the analytical method of taurine by high performance liquid chromatograph and detect the taurine content in different seafood tissues.[Method] The pre-column derivatization of o-phthalaldehyde (OPA) was used to detect the taurine by studying the effects of flow phase ratio,flow rate,ratio of derivatization agent and derivatization time.[Result]The linear range (R2 ＞ 0.99)was in the range of 10-400 μg/mL at the taurine∶ OPA =1∶ 2,derivatization time 2 min,acetonitrile∶ water =15∶ 85,flow rate 0.5 mL/min,wavelength 360 nm.The relative standard deviation of the parallel samples was 2.9％-7.4％.The content of taurine in different seafood was tested by using this method.The results showed that taurine was determined in all selected marine shellfish,crustaceans and fish.The highest content of taurine was observed in the baby clam,which was up to 11.2 mg/g.Compared with different tissues of fish,the content of taurine in muscle was the higher,while the lower in gill.[Conclusion] The results of this study enrich the data of taurine content in marine life,and provide reference for the further study of the synthesis mechanism of taurine in different tissues.
关键词
海产品/高效液相色谱/牛磺酸Key words
Seafood/High performance liquid chromatography/Taurine引用本文复制引用
基金项目
浙江海洋大学人才引进科研启动项目(Q1420)
2016年国家级大学生创新创业训练计划项目(201610340005)
2016年舟山市科技局浙江海洋大学专项(2016C41002)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据