不同来源山羊体细胞重编程效率比较
Comparison of Somatic Cell Reprogramming Efficiency among Different Sources of Goats
宋辉 1李卉 1王锋2
作者信息
- 1. 宁夏医科大学生育力保持省部共建教育部重点实验室,宁夏银川750004;南京农业大学江苏省肉羊产业工程技术研究中心,江苏南京210095
- 2. 南京农业大学江苏省肉羊产业工程技术研究中心,江苏南京210095
- 折叠
摘要
[目的]检测克隆奶山羊(♀12003)和转基因克隆奶山羊(♂12001)作为供体细胞,对体细胞重编程效率的影响.[方法]采用慢病毒作为载体,携带多能因子来感染正常山羊(g2)、♀12003和♂ 12001体细胞,统计克隆形成率和碱性磷酸酶阳性克隆率.[结果]与普通山羊(g2)相比,转基因克隆奶山羊耳成纤维细胞的重编程效率低,出现的细胞克隆在传代之前容易分化;克隆奶山羊体细胞与普通山羊体细胞的重编程效率接近,转基因克隆奶山羊(♂12001)的重编程效率极显著低于克隆奶山羊(♀12003)和普通山羊(P＜0.01).[结论]转基因克隆奶山羊体细胞可以被重编程为诱导性多能干细胞(Induced pluripotent stem cells,iPSCs),但效率较低.
Abstract
[Objective] To study the effects of cloned dairy goat (♀ 12003) and transgenic cloned dairy goat (♂ 12001) as donor cells on somatic cell reprogramming efficiency.[Method]The lentivirus was used as carrier to carry the pluripotent factor to infect the somatic cells of normal goat (g2),♀12003 and ♂ 12001.And the clonal formation rate and alkaline phosphatase positive clone rate were recorded.[Result]Compared with the normal goat (g2),the reprogramming efficiency of ear fibroblasts of the transgenic cloned dairy goat was lower,and the cell clones appeared to be easily differentiated before passage.The reprogramming efficiency of cloned dairy goat's somatic cells was close to that of the normal goat's somatic cell.The reprogramming efficiency of transgenic cloned dairy goat (♂ 12001) was significantly lower than that of cloned dairy goat (♀ 12003) and common goat (P ＜ 0.01).[Conclusion] Transgenic cloned dairy goat's somatic cells could be reprogrammed into induced pluripotent stem cells (iPSCs),but the efficiency was low.
关键词
诱导性多能干细胞/重编程/克隆羊Key words
Induced pluripotent stem cells/Reprogramming/Cloned goat引用本文复制引用
基金项目
宁夏医科大学校级特殊人才启动计划项目(XT201402)
宁夏医科大学基础医学西部一流学科建设项目()
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据