油棕乙酰CoA羧化酶(ACC)基因的鉴定与表达分析
Identification and Expression Profile of Acetyl CoA Carboxylase (ACC) in Elaeis guineensis
肖勇 1雷新涛 1王永 1曹红星 1石鹏 1金龙飞 1夏薇1
作者信息
- 1. 中国热带农业科学院椰子研究所,海南文昌571339
- 折叠
摘要
[目的]对油棕乙酰CoA羧化酶(ACC)基因进行鉴定与表达分析.[方法]采用生物信息学方法鉴定油棕的ACC基因,并应用拟南芥的ACC基因蛋白质序列比对油棕的蛋白质库,应用已有油棕转录组信息,研究ACC基因在不同组织中的表达情况.[结果]在油棕中鉴定了2个ACC基因,命名为EgACC1和EgACC2.EgACC1不舍内含子,而EgACC2含有32个内含子;EgACC1在不同组织中都没有表达,而EgACC2在所有组织中都有表达,其中在组培苗中有较高的表达量,同时,在油棕果发育21 d时有高的表达水平,RPKM值为70.6,为所有组织中最高.[结论]该研究阐述了油棕ACC基因的表达模式,为油棕ACC基因的功能分析奠定了基础.
Abstract
[Objective]To study the identification and expression profile of acetyl CoA carboxylase (ACC) in Elaeis guineensis.[Method]To identify ACC genes in E.guineensis by using bioinformatics,ACC genes protein sequence ofArabidopsis thaliana were used to align with protein database of E.guineensis.The transcription group information of E.guineensis was applied to study the expression of ACCgenes in different tissues.[Result] Two ACC genes were identified in the genome of E.guineensis,which were named as EgACC1 and EgACC2,respectively.Among them,EgACC1 do not contain intron,however EgACC2 contain thirty two introns.The expression of EgACC1 was not detected in different tissues of E.guineensis.However,the expression of EgACC2 was detected in all tissues.High expression levels were examined in tissue culture seedling.Meanwhile,highest expression level was detected at 21 day of mesocarp development,RPKM value was 70.6.[Conclusion] These study demonstrated that the expression pattern of ACC genes and provided basic work for functional analysis of ACC genes in E.guineensis.
关键词
油棕/ACC基因/转录组/RPKMKey words
Elaeis guineensis/ACC gene/Transcriptome/RPKM引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据