中国青凤蝶3个亚种的遗传变异分析
Analysis of Genetic Variation in Three Subspecies of Graphium sarpedon Linnaeus from China
杨斯琦 1王亚楠 2王方方 2何娅 1靳亚丽1
作者信息
- 1. 上海科技馆上海自然博物馆自然史研究中心,上海200041
- 2. 中国农业科学院上海兽医研究所农业部动物寄生虫学重点实验室,上海200241
- 折叠
摘要
[目的]探讨中国青凤蝶(Graphium sarpedon Linnaeus)3个亚种的遗传变异.[方法]对青凤蝶3个亚种的COⅠ基因和EF-1α基因部分序列进行测定,并对序列的碱基组成、转换颠换数和遗传距离等进行分析.[结果]在测得的CO Ⅰ基因(709 bp)和EF-1α(1 064 bp)基因中,有44个变异位点,6个简约信息位点,CO Ⅰ基因A+T平均含量为69.3％,存在较强的含量偏向性,亚种间的遗传距离相差甚小.[结论]青凤蝶3个亚种之间没有明显序列差异,仍然适宜采用传统的基于形态学的青凤蝶亚种分类体系.
Abstract
[Objective] To investigate the genetic variation in three subspecies of Graphium sarpedon Linnaeus in China.[Method] The CO Ⅰgene and the EF-1 α gene were partially sequenced.The nucleotide composition,the number of transition and transversion,genetic distance of the segment had been analyzed.[Result]There were 44 variation sites and 6 parsimony-informative sites in combined sequence of the CO Ⅰgene(709 bp)and EF-1α(1 064 bp) gene in the examined species.The average A + T content of CO Ⅰ gene was 69.3％,which showed strong A + T bias.But genetic distance among subspecies were very small.[Conclusion] It has no significant sequence differences between the three subspecies,which is still suitable to use the traditional morphological classification system for Graphium sarpedon subspecies.
关键词
青凤蝶/COⅠ基因/EF-1α基因/遗传变异Key words
Graphium sarpedon/COⅠ gene/EF-1α gene/Genetic variation引用本文复制引用
基金项目
上海市金山区水务局专项()
上海科技馆种子资金项目()
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据