9种植物精油及精油单体成分对黄瓜枯萎病的抑菌活性
Antifungal Activity of Nine Essential Oils and Essential Oil Monomers against Fusarium oxysporum f.sp.cucumerinum
贾会玲 1黄晓德 2钱骅 2朱羽尧 2陈斌 2韩双双 1赵伯涛1
作者信息
- 1. 南京师范大学生命科学学院,江苏南京210023;南京野生植物综合利用研究院,江苏南京210042
- 2. 南京野生植物综合利用研究院,江苏南京210042
- 折叠
摘要
[目的]研究9种植物精油及精油单体成分对黄瓜枯萎病的抑菌活性.[方法]采用滤纸片法和试管梯度稀释法测定了9种精油及精油单体成分对黄瓜枯萎病的抑菌活性.[结果]与阳性对照福美双相比,异硫氰酸丙烯酯和肉桂精油对黄瓜枯萎病的抑制作用较强,抑菌圈直径分别达(32.0±0.3)和(20.4±0.2)mm.异硫氰酸丙烯酯对黄瓜枯萎病的最小抑菌浓度(MIC)与最小杀菌浓度(MFC)均为0.049 μL/mL,肉桂精油对黄瓜枯萎病菌的MIC和MFC均为0.098 μL/mL,其余精油及精油单体的抑菌效果较弱.[结论]异硫氰酸丙烯酯和肉桂精油对黄瓜枯萎病的抑菌活性较好,具有进一步研究的价值.
Abstract
[Objective] The antifungal activity of nine essential oils and essential oil monomers was tested against Fusarium oxysporumf.sp.cucumerinum.[Method] The antifungal activity of nine essential oils and essential oil monomers was studied by using filter paper-research and broth dilution method.[Result] The inhibition zone of allyl isothiocyanate and cinnamon essential oil on Fusarium oxysporumf sp.cucumerinum were (32.0 ± 0.3) and (20.4 ± 0.2) mm,respectively,compared with the positive control.The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) on Fusarium oxysporum f.sp.cucumerinum of the allyl isothiocyanate were both 0.049 μL/mL,and the MIC and MFC on Fusarium oxysporum f.sp.cucumerinum of the cinnamon oil were both 0.098 μL/mL.Their antifungal effect was close to thiram.But the inhibition effects of the rest of essential oils and essential oil monomers were weaker.[Conclusion]Allyl isothiocyanate and cinnamon essential oil had better antifungal effects,which was important to further research and develop.
关键词
植物精油/精油单体成分/黄瓜枯萎病/抑菌活性Key words
Essential oil/Essential oil monomers/Fusarium oxysporum f.sp.cucumerinum/Antifungal activities引用本文复制引用
基金项目
江苏省政策引导类计划(产学研合作)—前瞻性联合研究项目政策引导类计划项目(BY2016016-01)
国家自然科学青年基金(31501462)
江苏省自然科学青年基金(BK20150067)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据