石蜡切片方法的改良
Method Improvement of Paraffin Section
赵亚林 1闫青地 1冯佳佳 1肖锋 1杨卓 1王凤茹 1董金皋1
作者信息
- 1. 河北农业大学生命科学学院/河北省植物生理与分子病理学重点实验室,河北保定071001
- 折叠
摘要
[目的]快速且安全高效制作石蜡切片.[方法]采用不同的方法,对植物材料进行透明和脱蜡以及包埋处理,把材料制成透明的玻片标本,利用正丁醇代替二甲苯作为透明剂和脱蜡剂,其他和传统的石蜡切片方法相同.[结果]正丁醇既能与乙醇混合,又能与中性树胶相融合;正丁醇作用相对较缓和,透明后的组织平整,没有明显的收缩、变形现象,并且不存在透明时间过长的问题,还减少了对实验人员的伤害.[结论]该方法可以快速地制作石蜡切片,且安全有效.
Abstract
[Objective] The aim was to make paraffin section safely and fast.[Method] Different methods were used to transparent and dewax the plant material and embedding,and the material was made into a transparent glass slide,and the n-butanol could instead of xylene.The other steps were the same as the traditional paraffin section method.[Result] The n-butanol not only could be mixed with ethanol,but also could be mixed with neutral gum.The n-butanol was relatively gentle,could obtain transparent tissue after formation,had no obvious shrinkage,deformation and transparent problem of long time,and reduced the damage to experimental staff.[Conclusion] The method could make paraffin section safely and fast.
关键词
石蜡切片/透明剂/正丁醇/花Key words
Paraffin section/Clearing agent/n-butanol/Flower引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据