响应面法优化八月瓜果皮总多酚的超声提取工艺
Optimization on Ultrasonic Extraction Technology of Total Polyphenol in Akebia trifoliate Pericarp by Response Surface Method
符智荣 1李雪峰 1周登维 2张晓旭 2欧阳玉祝2
作者信息
- 1. 吉首大学师范学院,湖南吉首416000
- 2. 吉首大学化学化工学院,湖南吉首416000
- 折叠
摘要
[目的]优化八月瓜果皮超声水解提取总多酚的工艺条件.[方法]以八月瓜果皮为研究对象,通过单因素试验和响应面试验考察了水解pH、水解温度和水解时间对总多酚得率的影响,建立了数学模型.[结果]用乙醇作为溶剂,按1∶20(g/mL)固液比加60％乙醇水溶液,在最优条件下(pH 5.23,水解温度53.76℃,水解时间115.66 min),模型对总多酚得率的预测值为5.63％.优化条件组合(pH 5.23,水解温度54℃,水解时间116min)试验下总多酚得率为5.58％,模型预测值与组合试验结果吻合.[结论]试验结果为八月瓜的开发提供了理论依据.
Abstract
[Objective] The aim was to optimize extraction technology of total polyphenol by ultrasonic-hydrolysis.[Method] With Akebia trifoliate pericarp as the research object,Influence of pH,hydrolysis temperature and time on extraction yield of total polyphenol was investigated by single factor experiment and response surface method.Mathematical model of the experiment was established.[Result] The results show that using ethanol as solvent,according to 1:20 (g/mL) solid-liquid ratio addition 60％ ethanol aqueous solution,model on predictive value of total polyphenols extraction yield was 5.63％ at pH of 5.23,53.76 ℃ hydrolysis 115.66 min.Extraction ratio of total polyphenols under combination experiment of optimal condition was 5.58％.The model predicted values and the combination experiment result was identical.[Conclusion] The results provide reference for the exploitation of Akebia trifoliate.
关键词
八月瓜/总多酚/水解/响应面法Key words
Akebia trifoliate/Total polyphenol/Hydrolysis/Response surface method引用本文复制引用
基金项目
湖南省湘西自治州科技计划项目(州财企函[2013]7号)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据