半微量凯氏法测定植株中全氮及测量不确定度评定
Determination of Total Nitrogen in Plant Samples by Semimicro-Kjeldahl Method and Uncertainty Evaluation of Measurement Results
刘丽敏 1顾重武 1曾燕燕1
作者信息
- 1. 浙江省河海测绘院,浙江杭州310010
- 折叠
摘要
[目的]测定植株中全氮并对测量不确定度进行评定.[方法]以浓H2SO4和H2O2为消煮液,采用优化后的升温程序,用半微量凯氏法测定植株中全氮.[结果]通过对植株样品和标准植株样的测定及不确定度评定可知,测定数据可靠,结果准确合理.[结论]测定过程中的不确定度主要来源于盐酸标准溶液和试样测定重复性,为实验室准确快速测定植株中全氮提供参考.
Abstract
[Objective] Total nitrogen in plant was measured by semimicro-kjeldahl method and the uncertainty of measurement results was evaluated.[Method] Total nitrogen was measured with concentrated sulfuric acid and hydrogen peroxide as boiling liquid at the optimized heating program.[Result]The uncertainty of measurement of plant samples and standard plant samples showed that the data was reasonable and the results were accurate and reliable.[Conclusion] Main sources of the total uncertainty came from the hydrochloric acid standard solution,titration process and repetitive titration.It could be reference for laboratory accurate and rapid determination of total nitrogen in plant.
关键词
半微量凯氏法/全氮/不确定度评定/植株Key words
Semimicro-Kejeldahl method/Total nitrogen/Uncertainty evaluation/Plant引用本文复制引用
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据