一种基于TaqMan探针法的阪崎肠杆菌qPCR全自动检测技术体系的建立
Establishment of qPCR Automatic Detection System Based on TaqMan Probe Method for Enterobacter sakazakii
王芬 1刘宽 2马龙 2李阳 1吴酬飞1
作者信息
- 1. 湖州师范学院生命科学学院,浙江湖州313000
- 2. 浙江农林大学林业与生物技术学院,浙江杭州310000
- 折叠
摘要
[目的]建立快速准确地检测食物样品中食源性致病菌的体系.[方法]配套湖州市微生物制剂与农产品安全重点实验室自主研发的微生物分子检测仪,建立一种基于TaqMan探针法的阪崎肠杆菌qPCR检测体系.[结果]体系建立成功,标准曲线斜率为-3.44,R2 =0.995 5,扩增效率为95.3％.[结论]该qPCR体系特异性强,灵敏度高,明显缩短了检测时间,并具有良好的稳定性与重复性,为食品中阪崎肠杆菌的高通量和自动化检测提供了一个新的途径.
Abstract
[Objective]To establish a rapid and accurate detection system for foodbome pathogens in food samples.[Method] A qPCR detection system for Enterobacter sakazakii based on TaqMan probe method was established,which was supported by the microbial molecular detector researched by key laboratory of Huzhou mocrobilogical agents and agricultural products safety.[Result]The system was successfully established and the standard curve slope,R2,amplification efficiency was-3.44,0.995 5,95.3％,respectively.[Conclusion] The qPCR detection system has strong specificity,high sensitivity,good stability and repeatability,which could shorten the detection time greatly.The system provides a new way for high throughput and automated detection of E.sakazakii in food.
关键词
阪崎肠杆菌/qPCR/全自动检测Key words
Enterobacter sakazakii/qPCR/Automatic detection引用本文复制引用
基金项目
浙江省食药监管系统科技计划项目(SP201618)
浙江省大学生科技创新活动计划(新苗人才计划)项目(2016R427009)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据