适用于转录组测序的大豆根总RNA的提取方法研究
Study on Methods to Extract High-quality Total RNA from Soybean for RNA-Seq
谢钰珍 1覃鸿妮 1张宇 2梅啸 1吴凡1
作者信息
- 1. 苏州工业园区服务外包职业学院,江苏苏州215123
- 2. 金唯智(苏州)生物科技有限公司,江苏苏州215123
- 折叠
摘要
[目的]获得高质量、高产量并能满足转录组测序要求的大豆根总RNA.[方法]比较分析了Trizol法、CTAB-LiCl法和试剂盒法的提取效果.[结果]3种方法均能从大豆根中提取出总RNA.但与Trizol法、CTAB法相比,试剂盒法提取的大豆根总RNA纯度高、完整性好,平均浓度达413.9 ng/μL,A260/A280≈2.14,A260/A230≈2.10,方法可重复性好.[结论]试剂盒法提取的RNA满足转录组测序的质量要求,可进行后续的转录组测序和其他分子生物学相关研究工作.
Abstract
[Objective] To obtain total RNA from soybean roots with high quality,high yield and to meet the transcription group sequencing requirements.[Method]Three extracting effects were compared,including Trizol reagent,CTAB-LiCl method and Kit method.[Result] All three methods can extract total RNA from soybean.However,the extraction kit was the best with high purity and integrity RNA.The average concentration was 413.9 ng/μL,A260/A280 was approximately 2.14,A260/A230 was 2.10 by using extraction kit,which was repeatable.[Conclusion]The RNA extracted by Kit method meets the quality requirements for transcriptome sequencing and can be used for subsequent RNA-seq and other molecular biology-related studies.
关键词
大豆/根/总RNA/转录组测序/提取方法Key words
Soybean/Root/Total RNA/RNA-seq/Extraction method引用本文复制引用
基金项目
苏州工业园区服务外包职业学院校级科研项目(ky-cyzx602)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据