Effects of Different Epiphyllous Species on Dendrobine of Dendrobiun nobile
[Objective] To measure the contents of dendrobine of Dendrobiun nobile from different epiphyllous trees.[Method] D.nobile is a kind of plant found in Chishui of Guizhou Province,which attached to different epiphyllous trees to grow for one more year.Gas chromatography internal standarded method was adopted to determine the contents of Dendrobine.An DB-1 (30 m ×0.25 mm ×0.25 μm) column was used,with the programming temperature 80 ℃,and then up to 250 ℃ keeping 5 min with the increasing rate of 10 ℃.The injector temperature was 250 ℃ and the FID detector temperature was 250 ℃.[Result] The Dendrobine contents of D.nobile on Cunninghamia lanceolata was 0.932%,0.825% on loquat,0.790% on Myrica rubra,0.601% on Dimocarpus longan.[Conclusion] The contents of dendrobine of D.nobile on different epiphyllous trees can meet the requirement of Chinese Pharmacopoeia(2015),the research offered evidence of ideal habitat for the growth of D.nobile.