Isolation,Identification and Characterization of Culturable Microorganisms from Frass of Protaetia brevitarsis Larva
[Objective]To screen cultivable functional microorganisms,and provide microbial resources for the recycling of agricultural waste.[Method]Taking the frass of Protaetia brevitarsis larva with the edible fungi residues as the research object,the plate coating method was used to separate culturable microorganisms from feces,and their ability to decompose cellulose and protein was preliminarily explored.[Result]A to-tal of 5 strains of bacteria,and 3 strains of fungi were isolated.After identification,they were identified as Cellulosimicrobium cellulans,Entero-coccus casseliflavus,Arthrobacter protophormiae,Glutamicibacter halophytocola,Streptomyces griseolus,Candida tropicalis,Trichoderma longi-brachiatum,Geotrichum candidum.Among them,Cellulosimicrobium cellulans and Streptomyces griseolus had the ability to decompose protein,and the ratio of transparent circle diameter to colony diameter(HC)on casein culture medium were 7.259±0.417 and 1.971±0.584,re-spectively.Arthrobacter protophormiae,Streptomyces griseolus,Geotrichum candidum and Trichoderma longibrachiatum had the ability to degrade cellulose,and after Congo red staining on sodium carboxymethyl cellulose,the HC values of the transparent circle were 1.166±0.028,2.044±0.245,1.075±0.029 and 1.288±0.048.[Conclusion]Cellulosimicrobium cellulans,Arthrobacter protophormiae,Streptomyces griseolus,Geotrichum candidum and Trichoderma longibrachiatum can be used as strain resources for the reuse of agricultural wastes such as edible fungi residues.
Protaetia brevitarsisFrass of larvaCulturable microorganismsIsolation and identification
国家科技期刊平台
NSTL
万方数据
维普
