Study on the Identification of Limnotrachelobdella sinensis and the Germicidal Efficacy of Drug Immersion Bath
Limnotrachelobdella sinensis,a freshwater leech parasitic on the gills of Aristichthys nobilis and Hypophthalmichthys molitrix in natu-ral water body,was reported for the first time.During 2019-2022,a small number of larvae were found to be infected on the gills of A.nobilis and H.molitrix in some reservoirs after October each year.The infection peaked from April to June in the following year,and then disappeared after July.It absorbed the blood on the fish gills,which seriously affected the fish growth and market sales price,and brought huge economic los-ses to the fishery production.It was identified by morphology and molecule methods as L.inensis.The killing effect of several commonly-used drugs(trichlorfon,toosendan,quicklime and cupric sulfate)on L.sinensis was tested by dipping bath in vitro and in vivo(parasitized on the gills of H.molitrix).The in vitro test showed that soaking with 200.00 mg/L quicklime,400.00 mg/L toosendan and 4.00 mg/L trichlorfon for 1 hour could kill all L.inensis,while in vivo test showed that 400.00 mg/L toosendan,2.00 mg/L trichlorfon and 100.00 mg/L quicklime were soaked for 1 hour and then temporarily cultured in clean water,and all the parasites were killed for 24 hours and H.molitrix was safe.
Limnotrachelobdella sinensisImmersion bathGermicidal efficacyIn vitro testIn vivo test
国家科技期刊平台
NSTL
万方数据
维普
