Expression and Bioinformatics Analysis of Cadmium Stress Response Gene sbbHLH168 in Sorghum bicolor
[Objective]The study aimed to explore the molecular mechanism of cadmium stress tolerance in Sorghum bicolor gene sb-bHLH168,which plays an important role in plant response to biotic and abiotic stresses,it provides a theoretical reference for breeding cadmi-um-tolerant Sorghum bicolor.[Method]A significantly up-regulated sbbHLH168 was screened from the transcriptome data(RNA-seq)of Sor-ghum bicolor cultivar'BTx623'under cadmium stress,and its biological information was analyzed by on-line software subcellular localization of sbbHLH168 gene was carried out by infection of onion epidermis,PGBKT7-sbbHLH168 vector was constructed to verify the transcriptional activation activity of sbbHLH168 gene,and Sorghum bicolor plants cultured in water were treated with NaCl,PEG,ABA,ACC,GA and JA solutions,RNA was extracted at 0,1,12,24 and 48 hours respectively,and the relative expression of sbbHLH168 gene was detected by RT-qPCR.[Result]The open reading frame length of sbbHLH168 gene was 567 bp,encoding 189 amino acid residues.The molecular weight of sb-bHLH168 protein was 20.55 kDa,and the theoretical isoelectric point(PI)was 8.86.sbbHLH168 was a basic protein with positive charge,which was located in the nucleus of cells,it has strong transcriptional activation activity.The expression level of sbbHLH168 was increased un-der salt,drought and plant hormone stress,but the expression level was significantly different at different treatment time.The expression of sb-bHLH168 gene in different tissues showed that sbbHLH168 gene was expressed in root and leaf of Sorghum bicolor,and the highest expression level was observed under salt,drought,ABA,GA and JA stress for 12 h.In ACC treatment,the highest expression level was observed at 24 h.[Conclusion]sbbHLH168 gene is a transcription factor with transcriptional activation activity located in the nucleus and belongs to bHLH family.
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