The Polymorphism Detection of MyoD Gene and Correlation Analysis with Growth Traits in Quail
[Objective]The purpose of this study was to explore the correlation between the different genotypes of MyoD gene and growth traits in quails.[Method]Korean quails were chosen as the research object,HRM technology was used to detect the polymorphism of MyoD gene in quails,and the growth traits were measured.The correlation analysis between growth traits and MyoD gene were analyzed.The mRNA expression of MyoD three genotypes were analyzed by qPCR in muscle tissue in embryonic stage of quail.[Result]The results showed that three genotypes(AA,Aa and aa)of MyoD gene were detected in the quail population.The chest circumference was significantly higher in AA and Aa genotype than aa genotype(P＜0.05).The results of qPCR showed that MyoD gene expression could be detected at 7-15 d of embryonic development,the level of MyoD gene expression was increased on day 11,and reached a peak on day 15 of embryonic development.The expression level of AA and Aa genotypes was significantly faster than that of aa genotype.[Conclusion]It is speculated that A allele of MyoD gene may be the domi-nant genotype during the embryonic growth and development of Korean quail,and can be used to genotype selection for rapid growth in quail breeding.
国家科技期刊平台
NSTL
万方数据
维普
