Effects of Different Litter Leaves and Phosphorus Addition on Soil Organic Carbon and Nitrogen Components of Pinus massoniana Soil in Subtropics
[Objective]To investigate the effects of leaf litter,P addition,and their interactions on changes in SOC and SON components.[Method]The effects of three kinds of withered leaf additions(Michelia macclurei,Pinus masson and Liquidam fortiana)and P addition(KH2 PO4)on SOC and SON components were investigated through a 125-day indoor culture experiment with the soil of Pinus massoniana as the research object.[Result]The results showed that both leaf litter addition and P addition significantly reduced the content of recalcitrant C(RP-C)component,and they showed a strong interaction.The effects of leaf litter on SOC and SON were influenced by soil P availability,and P addition accelerated the decomposition of original recalcitrant C by soil microorganisms under leaf litter addition.Redundancy analysis showed that ammonia nitrogen(NH+4-N)and available phosphorus(AP)were the most important influencing factors of SOC fraction,while dissolved organic carbon(DON)and microbial biomass phosphorus(MBP)were the most important predictors of SON fraction.[Conclusion]P addition can promote the decomposition and transformation of leaf litter in soil,and the input of leaf litter increases the decomposition of recalcitrant C,which will further accelerate the turnover of SOC.This study will provide a scientific basis for the study of carbon and nitrogen cycling in sub-tropical soil with increased litter input and P limitation and forest soil management in Daiyunshan Nature Reserve.
Leaf litterPhosphorus additionTwo-step acid hydrolysisCarbon and nitrogen componentsEnzyme activity