Characteristics of Rhizosphere Soil Microbial Community Structure and Its Effects on Secondary Metabolites of Notopterygium inci-sum
[Objective]To scientifically evaluate the health status of the soil ecosystem in the main production areas of N.incisum,and reveal the interaction relationship between soil microorganisms and N.incisum.[Method]Taking the rhizosphere soil of N.incisum as the research ob-ject,high-throughput sequencing technology and high-performance liquid chromatography(HPLC)were used to determine the composition of soil microbial community structure and the content of secondary metabolites of N.incisum.Furthermore,redundancy analysis and correlation a-nalysis methods were used to study the impact of rhizosphere soil microbiota on secondary metabolites of N.incisum.[Result]The results showed that the dominant bacterial at the phylum level were Proteobacteria,Acidobacteria and Bacteroidetes,and the Ascomycota,Basidiomyco-ta and Glomeromycota were identified as the dominant phyla of fungi.At the genus level,the dominant genera of soil bacteria were Vicinami-bacter,Terrimonas and nitrospira,which accounted for 0.80%-9.11%,0.66%-7.72%and 1.18%-4.462%,respectively.For fungi,the dominant genera were Mortierella,Tetracladium and Kotlabaca,which accounting for 0.01%-14.19%,0.18%-14.32%and 0.01%-24.96%,respectively.Redundancy analysis showed that the microbial community structure of the rhizosphere soil of N.incisum was mainly affected by soil pH and nutrient content.Colletotrichum and Botryichum showed significant negative correlation with pH(P＜0.05),while Bradyrhizobium also showed significant negative correlation with organic matter(P＜0.05).Candidatus_Udaeobate,Scutellospora and Enterocarpus were signif-icantly positively correlated with available phosphorus and available nitrogen(P＜0.05).The notopterol content were significantly negatively correlated with Chaetomium,Inocybe and Melanophyllum(P＜0.05),respectively,while isoimperatorin content was significantly positively correlated with Chaetomium and Humicola(P＜0.05),respectively.[Conclusion]The results of this study can provide data support for scientif-ic assessment of soil ecosystem characteristics and microbial-N.incisum interactions in the main N.incisum production area.
Notopterygium incisumRhizosphere soilMicrobial communityStructural characteristicsHigh-throughput sequencingPhysical and chemical factorsSecondary metabolites
国家科技期刊平台
NSTL
万方数据
维普
