HPLC法同时测定不同产地新疆鼠李枝中3种黄酮类成分的含量
Simultaneous Determination of Three Flavonoids in the Branches of Rhamnus songorica from Different Regions of Xinjiang by HPLC
叶斯木·塔拉甫别克 1叶力夏提·达那别克 1郭君婷 1赵婷婷 1刘桂花1
作者信息
- 1. 新疆维吾尔自治区药物研究所,新疆乌鲁木齐 830004
- 折叠
摘要
[目的]建立同时测定新疆鼠李枝中槲皮素、山奈酚、芹菜素的HPLC法,同时优化新疆鼠李枝提取工艺并测定其含量.[方法]采用Agilent XDB-C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇(A)-0.4%磷酸(B)为流动相,进行梯度洗脱(0～15 min,65%～55%B;15～35 min,55%～55%B;35～60 min,55%～40%B;60～70 min,40%～20%B;70～80 min,20%～65%B);检测波长360 nm,流速1 mL/min;柱温30℃.[结果]槲皮素、山奈酚、芹菜素分别在6.89～110.20、6.81～108.90、5.40～108.10 μg/mL线性关系良好(r≥0.999 6).新疆鼠李枝中上述3种成分的平均回收率分别为 100.00%、99.01%和 100.43%,RSD分别为 1.26%、1.76%、0.97%.[结论]该方法准确、可靠、重复性好,为新疆鼠李树枝提取物的质量评价与控制标准提供了重要依据.
Abstract
[Objective]To establish an HPLC method for the simultaneous determination of quercetin,kaempferol and apigenin in Rhamnus songorica branches,and to optimize the extraction process of Rhamnus songorica branches and determine their content.[Method]The HPLC a-nalysis was carried out on Agilent XDB-C18 column(4.6 mm×250 mm,5 μm)with methanol(A)-0.4%phosphoric acid(B)gradient elu-tion(0-15 min,65%-55%B;15-35 min,55%-55%B;35-60 min,55%-40%B;60-70 min,40%-20%B;70-80 min,20%-65%B);ata-flow rate of 1 mL/min.The detection wave-length was 360 nm and the column temperature was 30℃.[Result]Good linear relationship were found in the the range of 6.89-110.20 μg/mL for quercetin,6.81-108.90 μg/mL for kaempferol,and 5.40-108.10 μg/mL for apigeni(r≥0.999 6).The average recovery rates of the above three components in the branches of Rhamnus songorica were 100.00%,99.01%and 100.43%,respectively,with RSDs of 1.26%,1.76%and 0.97%.[Conclusion]This method is accurate,reliable,and has good repeatability,providing an important basis for the quality evaluation and control standards of Rhamnus songorica branch extract.
关键词
新疆鼠李/高效液相色谱法/槲皮素/山奈酚/芹菜素/含量测定Key words
Rhamnus songorica/HPLC/Quercetin/Kaempferol/Apigeni/Content determination引用本文复制引用
基金项目
新疆维吾尔自治区公益性科研院所基本业务费专项资金项目(KY2019105)
出版年
2023
国家科技期刊平台
NSTL
维普
万方数据