Simultaneous Determination of Three Flavonoids in the Branches of Rhamnus songorica from Different Regions of Xinjiang by HPLC
[Objective]To establish an HPLC method for the simultaneous determination of quercetin,kaempferol and apigenin in Rhamnus songorica branches,and to optimize the extraction process of Rhamnus songorica branches and determine their content.[Method]The HPLC a-nalysis was carried out on Agilent XDB-C18 column(4.6 mm×250 mm,5 μm)with methanol(A)-0.4%phosphoric acid(B)gradient elu-tion(0-15 min,65%-55%B;15-35 min,55%-55%B;35-60 min,55%-40%B;60-70 min,40%-20%B;70-80 min,20%-65%B);ata-flow rate of 1 mL/min.The detection wave-length was 360 nm and the column temperature was 30℃.[Result]Good linear relationship were found in the the range of 6.89-110.20 μg/mL for quercetin,6.81-108.90 μg/mL for kaempferol,and 5.40-108.10 μg/mL for apigeni(r≥0.999 6).The average recovery rates of the above three components in the branches of Rhamnus songorica were 100.00%,99.01%and 100.43%,respectively,with RSDs of 1.26%,1.76%and 0.97%.[Conclusion]This method is accurate,reliable,and has good repeatability,providing an important basis for the quality evaluation and control standards of Rhamnus songorica branch extract.
国家科技期刊平台
NSTL
万方数据
维普
