Optimization of High Efficiency Regeneration and Genetic Transformation System in Potato
In order to optimize the efficient regeneration and genetic transformation system of potato,the effects by using stem segment of Hu-bei potato No.3 as explants were studied in different hormone concentration ratios,and the optimal callus and differentiation hormone ratio were obtained.Then,the genetic transformation of Agrobacterium tumefaciens was used for screening to obtain the best transformation system,which provided the basis for genetic engineering breeding of high quality potato varieties.The results showed that the callus and differentiation rate were great different under the ratio of different hormone concentration.The hormone ratio of 2.0 mg/L ZR and 0.1 mg/L IAA could re-generate the buds efficiently,the germination rate was as high as 100%,and the bud growth was strong.By constructing expression vector pCambia1301 in plant,GV3101-mediated strain,pre-cultured for 2-3 days,and co-cultured for 2-3 days,and 350 mg/L carbenicillin,and 50 mg/L kanamycin to screen the concentration was better.The transformation efficiency could reach 65%.The transformed seedlings could form regenerated plants in the medium of 0.8 mg/L IBA and 0.2 mg/L NAA hormone.The regeneration rate could reach 80%,the stems were thick and the roots were developed.
万方数据
维普
