栝楼根腐病镰孢菌拮抗菌SJ1623发酵代谢物的抑制活性

Inhibitory Activity of Fermentation Metabolites of Antagonistic Bacteria SJ1623 Against Trichosanthes kirilowii Fusarium Root Rot

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中文摘要：检测了6株拮抗菌发酵上清液对栝楼根腐病菌的抑制活性,采用菌丝生长速率法测定优势拮抗菌不同接种量和发酵时间对发酵上清液抑菌活性的影响,测定了不同稀释倍数的发酵上清液和脂肽粗提物对栝楼根腐病菌的抑制活性.结果表明,对栝楼根腐病菌抑制活性最高的拮抗菌为SJ1623,种子液培养时间为10 h,0.75%(体积比)的接种量效果较好;发酵上清液稀释 10 倍对病菌的抑制率为87.6%,脂肽粗提物50倍稀释液对病菌的抑制率为83.1%.

外文摘要：The inhibitory activity of the fermentation supernatant of 6 antagonistic bacteria against Trichosanthes kirilowii Fusarium root rot was detected.The effects of different inoculation amount and fermentation time of the dominant antagonistic bacteria on the antifungal activity of fer-mentation supernatant were determined by the mycelial growth rate method.The inhibitory activity of fermentation supernatant and crude li-popeptide extract against Fusarium sp.was determined.The results showed that the antagonistic bacteria SJ1623 had the highest inhibitory ac-tivity.The seed liquid culture time was 10 h,and the inoculation amount of 0.75%(volume ratio)was better.The inhibition rate of the fer-mentation supernatant diluted 10 times was 87.6%.The inhibition rate of 50 times dilution of crude lipopeptide extract was 83.1%.

外文关键词：

Trichosanthes kirilowii root rotAntagonistic bacteriaFermentation brothInhibitory activity

作者：

余利、孟祥涛、陈甦、潘怀松、段海明

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作者单位：

安徽科技学院,安徽凤阳 233100

关键词：

栝楼根腐病拮抗菌发酵液抑制活性

基金：

凤阳县科技计划项目作物抗逆育种与减灾国家地方联合工程实验室开放基金安徽省高校自然科学研究项目安徽省高校自然科学研究项目安徽省重点研究与开发计划项目

项目编号：

NY2022-05NELCOF20190102KJ2017A510KJ2019A0814202104a06020001

出版年：

2024

DOI：

10.3969/j.issn.0517-6611.2024.03.028

安徽农业科学

安徽省农业科学院

安徽农业科学

影响因子：0.413

ISSN：0517-6611

年,卷(期)：2024.52(3)

参考文献量11