The inhibitory activity of the fermentation supernatant of 6 antagonistic bacteria against Trichosanthes kirilowii Fusarium root rot was detected.The effects of different inoculation amount and fermentation time of the dominant antagonistic bacteria on the antifungal activity of fer-mentation supernatant were determined by the mycelial growth rate method.The inhibitory activity of fermentation supernatant and crude li-popeptide extract against Fusarium sp.was determined.The results showed that the antagonistic bacteria SJ1623 had the highest inhibitory ac-tivity.The seed liquid culture time was 10 h,and the inoculation amount of 0.75%(volume ratio)was better.The inhibition rate of the fer-mentation supernatant diluted 10 times was 87.6%.The inhibition rate of 50 times dilution of crude lipopeptide extract was 83.1%.
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