Study on Influencing Factors of the Sclerotiums Germination in Laboratory Artificial Culture on Soybean
The artificial breeding sclerotiums were used for germination test.The germination difference between artificial breeding sclerotiums and natural sclerotiums,the influence of sclerotiums of several generations funguses and preservation methods on the germination of sclerotiums was studied.The results showed that the germination time of sclerotiums cultured by PDA substrate was less,the natural sclerotiums germination time was the longest and germination rate was the lowest,the germination time of sclerotiums which culture for 15 days in PDA substrate was shorter,and there was no significant difference between 30 days and 45 days.There was no difference between sclerotiums from different age funguses groups.The germination time of fresh breeding sclerotiums was the shortest,and the longer the dry storage time,the longer the sub-sequent germination culture time.