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中性蛋白酶高温酶解条件下猪粪资源化利用中养分的变化规律

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以酶工程及酶动力学为技术依托及科学依据,将猪粪加玉米秸秆组合作为底物,添加2%中性蛋白酶,在生化反应釜中80 ℃酶解3 h,底物含水量降低至56.01%,同比下降2.34%;大分子有机质含量降低至63.79%,酶解后,A、B处理的底物有机质含量分别降低了14.19%、15.06%;植物可利用大量元素提高至6.87%,符合NY 525-2012有机肥标准;速效磷、速效钾、全盐、总腐殖酸呈下降趋势;蛔虫卵死亡率达到92%,种子发芽指数达到54%,达到有机肥腐熟标准.
Changes Rule of Nutrients During the Resource Utilization of Pig Manure Under High Temperature Enzymatic Hydrolysis with Neu-tral Protease
Based on enzyme engineering and enzyme kinetics as the technical support and scientific basis,the combination of pig manure and corn stover was used as the substrate,2%neutral protease was added,and the water content of the substrate was reduced to 56.01%at 80℃ for 3 h in a biochemical reactor,a year-on-year decrease of 2.34%.The content of macromolecular organic matter was reduced to 63.79%,and after enzymatic hydrolysis,the organic content of substrates treated A and B reached 14.19%and 15.06%,respectively.The use of a large number of elements in plants had been increased to 6.87%,which met the NY 525-2012 organic fertilizer standard.Available-available phosphorus,avail-able potassium,total salt and total humic acid showed a downward trend.The mortality rate of roundworm eggs reached 92%,and the seed ger-mination index reached 54%,which reached the standard of organic fertilizer rot.

Pig manureNeutral proteaseHigh temperature enzymatic hydrolysisOrganic fertilizerNutrientChange rule

戴鹏飞、孙权、解昊郡、王锐、蒋鹏

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宁夏大学农学院,宁夏银川 750021

猪粪 中性蛋白酶 高温酶解 有机肥 养分 变化规律

宁夏回族自治区重点研发计划宁夏回族自治区重点研发计划

2019BCF010012022BEG02004

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(4)
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