Study on the Insecticidal Activity and Mechanism of Essential oil of Asarum heterotropoides Against Bactrocera dorsalis
[Objective]To study the insecticidal activities of essential oil of Asarum heterotropoides against Bactrocera dorsalis by fumigation and touch-killing,and preliminarily determine the insecticidal mechanism of the essential oil of Asarum heterotropoides against Bactrocera dor-salis,aimed to find natural and effective botanical insecticides.[Method]The fumigation activity and touch-killing activity of the essential oil against Bactrocera dorsalis were determined by closed fumigation method and micro spot method,the effects of essential oil of Asarum heterotro-poides on acetylcholinesterase(AchE)and carboxylesterase(CarE)activities in Bactrocera dorsalis were determined by microplate method.[Result]In the insecticidal activity experiment,with the increase of the concentration of the essential oil of Asarum heterotropoides,the insecti-cidal effect was gradually enhanced,and with the extension of time,the toxicity was also gradually enhanced.In the fumigation experiment,when the concentration was 1 mL/L and the treatment time was 12 h,the corrected mortality rate reached the maximum of 100％.In the touch-killing experiment,when the concentration was 156.25 μL/L and the treatment time was 12 h,the corrected mortality rate reached the maxi-mum of 100％.The LC50 of Bactrocera dorsalis treated with fumigation and touch-killing for 12 h were 0.243 mL/L and 30.884 μL/L.After treating Bactrocera dorsalis with a fixed concentration of essential oil(156.25 μL/L)for different time,AchE and CarE in Bactrocera dorsalis were inhibited as a whole.After treating Bactrocera dorsalis with different concentrations of essential oil for 8 h,the AchE in worm showed in-duction effect at low concentration and inhibition effect at high concentration.The inhibitory effect of essential oil of Asarum heterotropoides on AchE in Bactrocera dorsalis increased with the increase of concentration.[Conclusion]The essential oil of Asarum heterotropoides has good fumi-gation and touch-killing activities against Bactrocera dorsalis,and has stronger touch-killing activity.The essential oil of Asarum heterotropoides has inhibitory effects on AchE and CarE in Bactrocera dorsalis.This study can provide theoretical basis for the research and development of nat-ural botanical insecticides.
万方数据
维普
