Extraction of Polysaccharide from Mycelia of Sanghuangporus lonicericola and Analysis of Oxidative Reducibility
By selecting extraction method and determining the content of polysaccharide of S.lonicericola,the polysaccharide extraction meth-od with high extraction rate and the optimal conditions were selected.The results showed that ultrasonic assisted enzymatic method had a higher yield than water bath assisted solvent extraction method and microwave-assisted extraction method.The optimal extraction conditions were solid-liquid ratio of 1∶20(g∶mL),ultrasonic extraction time of 40 min,ultrasonic power of 300 W,the enzyme dosage of 0.15％.Under these conditions,the yield of polysaccharide from mycelial of S.lonicericola was(6.58±0.01)％.By HPLC external standard method,the main monosaccharide components of S.lonicericola mycelial polysaccharide were mannose,glucosamine,ribose,rhamnose,glucuronic acid,galac-turonic acid,amino galactose,glucose,galactose,xylose,arabinose and fucose.Through oxidation-reduction analysis of S.lonicericola myce-lial polysaccharide,it was found that S.lonicericola mycelial polysaccharide had certain antioxidant capacity,but compared to VC,the antioxi-dant effect of S.lonicericola mycelial polysaccharide was slightly weaker.
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